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Abstracts
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PLASMA PROCESSED SURFACES FOR SELECTED CELL RESPONSES
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P. Favia, E. Sardella, R. Gristina, M. Nardulli, L.C. Lopez, E.
Dilonardo, B. Pistillo, R. d’Agostino |
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Department of Chemistry, University of Bari, Italy
Institute of Inorganic Methods and Plasmas, CNR, Bari, Italy |
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Plasma deposited coatings and plasma treated surfaces can induce pre-determined
properties at the surface of polymers and other materials, that are desirable
for in vitro, ex vitro and in vivo biomedical applications such as biomaterials,
implantable prostheses, biosensors, tissue engineering and biomedical devices.
This talk will describe plasma functionalization processes that have been
optimized for obtaining bacterial-resistant coatings, functional surfaces
for biomolecule immobilization and surfaces with selected acid/basic character,
that have been utilized to induce selected responses by cells and bacteria. |
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Developments of Ionchannel Biosensor and Plasma Process
「イオンチャンネルバイオセンサーとプラズマプロセスの開発」
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Tsuneo Urisu
宇理須恒雄 |
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Institute for Molecular Science/SOKENDAI
分子科学研究所/総合研究大学院大学 |
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The membrane protein such as ion channel and GPCR plays an important role
as a receptor of the information in vivo transmission. It accounts for
50% or more of all drug development targets, because it takes part in almost
all diseases called an intractable disease. However, since there is no
excellent high-through-put screening biosensor for the membrane protein,
this makes the research and developments of new drugs difficult. In this
work, we have developed a planer type ion channel biosensor using a Si
substrate with micro pore、where TRPV1–channel-expressed HEK293 cell is
positioned. Ligand gated ion channel currents have been successfully observed
using capsaicin as a ligand molecue. Because the micro-fabrication of the
Si substrate is the most important process, the related plasma process
is described. |
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膜タンパクは、生体内の情報伝達の受信素子として重要な役割を果たしており、殆どの、難病と言われる疾患に関与しているため、 全創薬ターゲットの50%以上を占める。 しかし、高性能な、ハイスループットスクリーニング用のバイオセンサーが無いため、創薬研究開発を困難にしている大きな原因の一つである。 本研究では、膜タンパクであるイオンチャンネルのバイオセンサーとして、HEK293
細胞にTRPV1チャンネルを発現し、これを微細貫通孔を有するSi基板に搭載したプレーナー型イオンチャンネルバイオセンサーを開発し、カプサイシンのリガンド刺激電流の検出に成功した。 Si基板の微細加工が最も重要な工程として、関連のプラズマプロセスについて述べる。 |
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Nano-carbon Films Formed with Electron Cyclotron Resonance (ECR) Sputtering
and Their Application for Bioanalysis and Biosensors
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Osamu Niwa |
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National Institute of Advanced Industrial Science and Technology (AIST)
Division of Biological Resources and Functions
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The carbon thin films deposited by electron cyclotron (ECR) sputtering
method have unique structure and properties. The films consist of sp3 and
sp2 mixed bonds and have equivalent mean scratch hardness to the diamond.
The structure of the film from TEM image is different from usual graphite
carbon. The content of sp3 bonds increases with increasing acceleration
voltage during sputtering. As an electrode for electroanalysis, the film
shows wide potential window and low capacitive current and therefore applied
for measuring various biomolecules with high oxidation potentials including
histamine and NADPH and nucleotides. We prepared biosensor for detecting
-aminobutyric acid (GABA) which is a well know inhibitory neutrotransmitter
by modifying ECR sputtered carbon film with enzyme layer. The sensor shows
a low detection limit which is more than 1 order of magnitude of lower
than that of biosensor based on the conventional glassy carbon (GC) electrode.
With ECR sputtered carbon film electrodes, we also succeeded in determining
the oxidation peak potentials for 4 kind of nucleotides (GMP:1.23V, AMP:1.57V,
TMP:1.78V, CMP:1.99 V). In addition, the content of bases in oligonucleotide
can be roughly measured by differential pulse voltammetry (NPV) using the
ECR sputtered carbon film electrodes since the decrease in the oxidation
currents caused by the adsorption of oligonucleotide can be suppressed
due to weak interaction between the analytes and electrode surface. |
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Near field Raman spectroscopy for molecular nano-imaging and nano-
analysis
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Yasushi Inouye |
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Graduate School of Frontier Biosciences, Osaka University |
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Plasomon polaritons, collective oscillation of electrons coupled with photons,
are generated in metallic nanostructures when light is incident on the
nanostructures. Then, photons are confined and enhanced in the vicinity
of the nanostructures. We proposed a metallic needle having a nano-apex
as nano-light source for microscopy having super resolving capability,
which is called as near field microscopy. In this presentation, we will
show Raman nanospectroscopy by using this microscopic technique for molecular
nano-imaging and nano-analysis. Furthermore we will discuss nonlinear effects
generated in near field Raman spectroscopy which provides us spatial resolution
exceeding 10 nm. |
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Molecular Dynamics Simulations of Cell Membrane Nanopores with DNA
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Motohiko Tanaka, and Yitzak Rabin |
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Electrostatic effects sometimes play important roles in structure formation
of biological systems, most notable of which is seen in a tight structure
of double helix DNA. Here we talk about our molecular dynamics study of
membrane nanopores with or without a single-stranded(ss) DNA inside [1,2].
The cell membrane is characterized by very low dielectric constant (2)
compared to that of surrounding water (80), which leads to dramatic phenomena
due to electrostatic effects, including exclusion of isolated charged ions
and/or paring of salt ions in nanopores. A ss-DNA takes a stretched configuration
in the nanopore due to electrostatic repulsion from the pore wall. |
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References
[1] Y.Rabin and M.Tanaka, Phys.Rev.Lett., 94, 148103 (2005).
[2] M.Tanaka and Y.Rabin, Flow Dynamics, pp.212-216 (AIP CP832, 2006).
Also, refer to http://dphysique.nifs.ac.jp/
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Construction of Patterned Cell Spheroid Array for High Performance Cell
Based Biosensor
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Yukio Nagasaki |
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Tsukuba Research Center for Interdisciplinary Materials Science (TIMS),
and Center for Tsukuba Advanced Research Alliance (TARA),
University of Tsukuba, Ten-noudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan |
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In order to create high performance biofunctionality devices such as protein,
DNA and cell array chips, it is important to control an adsorption of biomolecules
and cells on substrate surface. The objective of this work was to construct
patterned cell culture system on precisely designed micropattened biointerface,
which was constructed by our original surface modification method using
PEG derivatives. The constructed patterned cell spheroid array, using primary
hepatocyte celle is promising as new cell based biosensor. |
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Katsuhisa Kitano and Satoshi Hamaguchi |
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Center for Atomic and Molecular Technologies, Graduate School of Engineering,
Osaka University |
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Many types of atmospheric pressure plasma jets (APPJs) have been developed.
At the atmospheric pressure, due to frequent collisions between high-energy
plasma particles and neutral gas, discharges can be easily transformed
to thermal plasmas (e.g., arc plasmas). Nevertheless nonthermal (nonequilibrium)
plasmas are also achieved by several techniques. From a viewpoint of plasma
applications to materials, such plasmas offer attractive opportunities
since nonthermal plasmas can supply chemically active species without heating
and/or damaging the materials to be processed. Characteristics of nonthermal
APPJs generated in and/or from a dielectric tube which is wrapped around
by two tubular electrodes connected to a low frequency (~10kHz) high voltage
power supply [M. Teschke, et al., IEEE. trans. Plasma Sci., 33, 310, (2005)]
and other related systems are discussed. The aim of this study is to understand
the mechanism of such nonequilibrium APPJ and also to extend the concept
for the development of new plasma generation systems at atmospheric pressure. |
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